Preparation of progesterone nanoparticles and evaluation of its effect on the capacitation of Bovine spermatozoa used in the in Vitro Fertilization

Saber Abd-Allah, Mai Ralan, Hanaa Suliman, Tamer Essam, Heba F Salem



Progesterone (P) has been reported to affect several sperm functions especially capacitation and acrosome reaction. The main problem of (P) is its low aqueous solubility. So formulation of progesterone nanoparticles (PN) will enhance its solubility. This study was conducted to produce nanosized progesterone (NP) and assess its biocompatibility. Therefore, nine progesterone formulations were prepared and characterized. Data analysis revealed only one formula of P showed nanosized particle (1-100 nm) with an average particle size (95±5 nm), and spherical shape as seen by Transmission Electron Microscope(TEM). Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with NP or P or without treatments (control) and incubated for 3h at 38°C and evaluated every 1 hour (h) interval. Ovarian oocytes were matured and fertilized in vitro with frozen-thawed bull sperm capacitated in vitro with NP or P or control (without NP, P) and incubated at 39C in 5% CO2 incubator for 24h and then examined for evidence of fertilization. In conclusion, this study demonstrates that nanosized progesterone is highly efficient for sperm capacitation. In addition to the use of nanosized progesterone in sperm capacitation produces more fertilized oocytes than the progesterone after In Vitro Fertilization (IVF).


Capacitation; In vitro fertilization; Nanoparticles; Progesterone; Spermatozoa.

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COVERING LETTER From: Hanaa Sayed Suliman Yousef Biotechnology Department, Faculty of Post graduate for Advanced Sciences, Beni-Suef University, Egypt. Telephone: +201117613536 Email: To: The Editor-in-Chief Subject: Manuscript Submission Article type: Original Article Dear Sir, Greetings, Please find attached soft copy of our manuscript entitled: (Preparation of progesterone nanoparticles and evaluation of its effect on the sperm capacitation of bovine spermatozoa used in the in vitro fertilization) for publication in your esteemed journal (International Journal of Drug Delivery). We here with confirm that the work described has not been published before; that is not under consideration for publication elsewhere. With agreement of all authors for publication in you journal I kindly request you to acknowledge the receipt of the same. I am looking forward to hearing from you. Thanking you. Sincerely yours Hanaa Sayed Suliman Date: 25/ 11 / 2017


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Copyright (c) 2017 Saber Abd-Allah, Mai Ralan, hanaa suliman, Tamer Essam, Heba Salem

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