Topical nanoemulsion of turmeric oil for psoriasis: characterization, ex vivo and in vivo assessment

Md Sajid Ali, Md. Sarfaraz Alam, Faisal Imam Imam, Masoom Raza Siddiqui


Psoriasis is a chronic; T lymphocyte mediated autoimmune inflammatory disorder characterized by well-defined erythematous (reddish) plaques with large adherent silvery scales that affects the skin and other parts of the body. The essential oil in turmeric is anti-inflammatory and effective in treating chronic disorders like psoriasis that have inflammation as a root symptom. Nanoemulsions are isotropic, thermodynamically stable transparent (or translucent) systems of oil, water, surfactant and co-surfactant with a droplet size usually in the range of 20–200 nm. Their long-term stability and ease of preparation (spontaneous emulsification) make it promising tool for drug delivery. The aim of this study was to obtain nanoemulsions of turmeric oil for psoriasis and to evaluate their physical stability, irritation potential and in vivo inflammatory activity. For the preparation of nanoemulsion titration method was used which was composed of 15% turmeric oil, 42 % Smix (1:1) and 43 % distilled water. The nanoemulsion was stable during the period of study and was found to be practically non-irritating in the organotypic HET-CAM model. The anti inflammatory activity of optimized nonoemulsion was carried out by carragennen induced paw edema and found to be 70.35 % inhibition.


Psoriasis; turmeric oil; nanoemulsion; anti-inflammatory; physical stability.

Full Text:



Alam MS, Ansari MS, Ali MD, Ali MS.Different approach for the treatment of psoriasis: a review. Inventi Impact: Clinical Research. Vol. 2012; Article ID- Inventi:pcr/139/12

Baboota S, Alam MS., Sharma S, Sahni JK, Kumar A, Ali J. Nanocarrier-based hydrogel of betamethasone dipropionate and salicylic acid for treatment of psoriasis. Int J Pharma Investig. 2011;1:139-47.

Christophers E. Psoriasis epidemiology and clinical spectrum.Clin Exp Dermatol. 2001; 26:314- 20.

Lo WY, Friedman JM.Teratogenicity of recently introduced medications in human pregnancy. Obstet Gynecol. 2002;100: 465-73.

Jayaprakasha GK, Jena BS, Negi PS, Sakariah KK. Evaluation of Antioxidant Activities and Antimutagenicity of Turmeric Oil: A Byproduct from Curcumin Production. Z Naturforsch C.2002;57:828–35.

Sandur SK, Pandey MK, Sung B, Ahn KS, Murakami A, Sethi G, Limtrakul P, Badmaev V, Aggarwal BB. Curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin and turmerones differentially regulate anti-inflammatory and anti-proliferative responses through a ROS-independent mechanism. Carcinogenesis. 2007;28(8):1765–73.

Srivastava R. Inhibition of neutrophil response by curcumin. Agents Actions. 1989;28(3-4):298- 303.

Srimal RC., Dhawan BN. Pharmacology of diferuloyl methane (curcumin), a non-steroidal anti-inflammatory agent. J Pharm Pharmacol. 1973; 25(6):447-52.

Aggarwal BB., Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases.The International Journal of Biochemistry & Cell Biology. 2009; 41(1): 40–59.

Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, et al. Comparative evaluation of 11 essential oils of different origins as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem. 2005;91:621–32.

Negi PS, Jayaprakasha GK, Rao JL, Sakariah KK. Antibacterial activity of turmeric oil: A byproduct from curcumin manufacture. J Agri Food Chem. 1999;47:4297–300.

Jayaprakasha GK, Jena BS, Negi PS, Sakariah KK. Evaluation of Antioxidant Activities and Antimutagenicity of Turmeric Oil: A Byproduct from Curcumin Production. Z Naturforsch C.2002;57:828–35.

Shah P, Bhalodia D, Shelat P. Nanoemulsion: A pharmaceutical review. Syst Rev Pharm. 2010;1: 24-32.

Shah D, Moudgil B, Mittal KL. Surfactants in Solution: Fundamentals and Applications, Surfactant Science Series.: New York: Marcel Dekker; 2002. 525–54.

Solans C, Izquierdo P, Nolla J, Azemar N, Garcia-Celma MJ.Nano-emulsions. Curr. Opin. Colloid Interface Sci. 2005; 10 :102–10.

Mei Z, Chen H, Weng T, YangY. Solid lipid nanoparticle and microemulsion for topical delivery of triptolide. Eur J Pharm Biopharm. 2003; 56:189–96.

Moshfeghi AA, Peyman GA. Micro- and nanoparticulates. Adv. Drug Deliv. Rev. 2005; 57:2047–52.

Rosen H, Abribat T.The rise and rise of drug delivery. Nat. Rev. Drug Discov. 2005; 4: 381–85.

Shah P, Bhalodia D, Shelat P. Nanoemulsion: A pharmaceutical review. Syst Rev Pharm. 2010;1:24-32.

Abdulrazik M, Tamilvanan S, Khoury K, Benita S. Ocular delivery of ciclosporin A. II. Effect of submicron emulsion’s surface charge on ocular distribution of topical cyclosporin. STP Pharma Science. 2001;11:427-32.

Gershanik T, Benita S. Positively charged self-emulsifying oil formulation for improving oral biovailability of progesterone. Pharm Devl Tech. 1996; 1:147-57.

Klang S, Benita S. Design and evaluation of submicron emulsion as colloidal drug carriers for intravenous administration. In: Submicron emulsions in drug targeting and delivery. Edited by: Benita S; 1998. 119-52.

Shafiq-un-Nabi S, Shakeel F, Talegaonkar S, Ali J, Baboota S, Ahuja A. Formulation development and optimization using nanoemulsion technique: A technical note. Pharm Sci Tech. 2007; 8: E12-E17.

Chen H, Chang X, Weng T, Zhao X, Gao Z, Yang Y, Xu H, Yang X. A study of microemulsion systems for transdermal delivery of triptolide. J Control Rel. 2004; 98: 427–36.

Shafiq S, Shakeel F, Talegaonkar S, Ahmad FJ, Khar R K, Ali M. Development and bioavailability assessment of ramipril nanoemulsion formulation. Eur Jr of Pharm and Biopharma. 2007; 66 : 227–43.

Bali V, Ali M, Ali J. Nanocarrier for the enhanced bioavailability of a cardiovascular agent: In vitro, pharmacodynamic, pharmacokinetic and stability assessment. Int Jr of Pharm. 2011; 403(1–2): 46–56.

Bernardi DS, Pereira TA, Maciel NR, Bortoloto J, Viera GS, Oliveira GC, Rocha-Filho P.A. Formation and stability of oil-in-water nanoemulsions containing rice bran oil: in vitro and in vivo assessments. JNanobiotechnology. 2011; 9: 44.

Attwood D. Microemulsions. In: Kreuer J, editor. Colloidal Drug Delivery Systems. New York: Marcel Dekker;1994.31–71.

Luepke N.P. Hen’s egg chorioallantoic membrane test for irritation potential. Food and Chemical Toxicology. 1985;23:287-91.

Mehling A, Kleber M, Hensen H. Comparative studies on the ocular and dermal irritationpotential of surfactants. Food and Chemical Toxicology. 2007; 45:747-58.

Al-Swayeh PK, Clifford RH, Del Soldato P, Moore PK. A comparison of the anti-inflammatory and antinociceptive activity of nitroaspirin and aspirin. Br J Pharmacol. 1999;129:343-50.

Winter CA. Anti-inflammatory testing methods: comparative evaluation of indomethacin and other agents. Int Symp Nonst Antiinflamm Drugs. 1965;82: 190-202.

Shakeel F, Baboota S, Ahuja A, Ali J, Shafiq S. Skin permeation mechanism and bioavailability enhancement of celecoxib from transdermally applied nanoemulsion. J Nanobiotechnology. 2008; 6: 1-11.

Kawakami K, Yoshikawa T, Moroto Y, Kanaoka E, Takahashi K, Nishihara Y, Masuda K. Microemulsion formulation for enhanced absorption of poorly soluble drugs. II. In vivo study. J Control Rel. 2002;81:75–82.

Kommuru TR, Gurley B, Khan MA, Reddy IK. Self-emulsifying drug delivery systems (SEDDS) of coenzyme Q10: formulation development and bioavailability assessment. Int J Pharm.2001;212:233–46.

Craig DQM, Barker SA., Banning D, Booth SW. An investigation into the mechanisms of self-emulsification using particle size analysis and low frequency dielectric spectroscopy. Int J Pharm. 1995;114:103–10.

Shinoda K, Lindman B. Organized surfactant systems: microemulsions. Langmuir. 1987; 3: 167– 80.

Mehling A, Kleber M, Hensen H. Comparative studies on the ocular and dermal irritationpotential of surfactants. Food and Chemical Toxicology. 2007; 45:747-58.

Wilson TD, Steck WF. A modified HET-CAM assay approach to the assessment of anti-irritant properties of plant extracts. Food and ChemicalToxicology 2000; 38:867-72.

Shakeel F, Baboota S, Ahuja A, Ali J, Faisal MS, Shafiq S. Stability evaluation of celecoxib nanoemulsion containing Tween 80. Thai Journal of Pharmaceutical Science. 2008; 32:4-9.

Shafiq Sand Shakeel F, Khar RK. Enhanced Stability of Ramipril in Nanoemulsion Containing Cremophor-EL: A Technical Note. AAPS PharmSciTech. 2008; 9(4): 1097-1101.

Masmoud H, Dreau YL, Piccerelle P, Kister J. The evaluation of cosmeticand pharmaceutical emulsions aging process using classical techniques and a new method: FTIR. International Journal of Pharmaceutics. 2005; 289:117- 31.



  • There are currently no refbacks.

Advanced Research Journals

4/70-71. Black Well HB, ALW 30100 IND 2009-2016


Follow @arjournals on Twitter