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Development and validation of an HPLC method for the quantification of a cytotoxic dihydropyranoxanthone in biodegradable nanoparticles


 
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1. Title Title of document Development and validation of an HPLC method for the quantification of a cytotoxic dihydropyranoxanthone in biodegradable nanoparticles
 
2. Creator Author's name, affiliation, country Maribel Teixeira; Centro de Investigação em Ciências da Saúde (CICS), Instituto Superior de Ciências da Saúde–Norte, CESPU. Rua Central de Gandra 1317, 4585-116 Gandra PRD, Portugal; Portugal
 
2. Creator Author's name, affiliation, country Ana Mafalda Paiva; 1Centro de Química Medicinal – Universidade do Porto (CEQUIMED-UP), 2Departamento de Ciências Químicas, Laboratório de Química Orgânica e Farmacêutica, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Portugal
 
2. Creator Author's name, affiliation, country Rosa Pereira; Centro de Química Medicinal – Universidade do Porto (CEQUIMED-UP), Portugal; Portugal
 
2. Creator Author's name, affiliation, country Carlos Maurício Barbosa; Centro de Química Medicinal – Universidade do Porto (CEQUIMED-UP), Departamento de Ciências do Medicamento, Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade do Porto, Portugal.; Portugal
 
2. Creator Author's name, affiliation, country Emília Sousa; Centro de Química Medicinal – Universidade do Porto (CEQUIMED-UP), Departamento de Ciências Químicas, Laboratório de Química Orgânica e Farmacêutica, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Portugal
 
2. Creator Author's name, affiliation, country Madalena M M Pinto; Centro de Química Medicinal – Universidade do Porto (CEQUIMED-UP), Departamento de Ciências Químicas, Laboratório de Química Orgânica e Farmacêutica, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Portugal
 
3. Subject Discipline(s) Pharmaceutical Sciences; Drug delivery
 
3. Subject Keyword(s) dihydropyranoxanthone; HPLC; polymeric nanoparticles; xanthones
 
4. Description Abstract

3,4-dihydro-12-hydroxy-2,2-dimethyl-2H,6H-pyrano[3,2-b]xanthen-6-one (compound 1) is a cytotoxic dihydropyranoxanthone exhibiting antiproliferative effects, inducing S-phase cell cycle arrest, and increasing the percentage of apoptotic cells in leukemia cell lines. Nevertheless, the poor aqueous solubility of compound 1 is a major drawback not only for its potential use in therapy but even for the in vitro assessment of its biological activity. Polymeric nanoparticles formulations were developed as potential carriers to overcome problems related with low water solubility of compound 1. The objective of this work was to develop and validate a specific, sensitive and simple HPLC method for the quantitative analysis of the prenylated xanthone (compound 1), which was entrapped in PLGA nanoparticles for the first time.

Chromatographic separation was performed with a reversed-phase C18 column, using methanol: water (85:15, v/v) containing 1 % (v/v) acetic acid as a mobile phase at a flow rate of 1 ml/min and quantification was made by UV detection at 254 nm. The isocratic system required 10 minutes of chromatographic run. The method was shown to be linear (r> 0.999) over the concentration range of 0.50-3.00 µg/ml and precise at the intra-day and inter-day levels as reflected by the relative standard deviation values (lower than 1.5% and 1.6%, respectively). The mean recovery ranged from 97.53 to 104.28 % (RSD: 0.027%) and from 98.49 to 101.81% (RSD: 0.019 %) for nanospheres and nanocapsules, respectively.

A simple, linear, sensitive, accurate, and precise HPLC method suitable for the quantification of compound 1 incorporated in polymeric nanoparticles was developed and validated.
 
5. Publisher Organizing agency, location Advanced Research Journals
 
6. Contributor Sponsor(s) FCT – Fundação para a Ciência e a Tecnologia and U.Porto/Santander Totta
 
7. Date (YYYY-MM-DD) 10-09-13
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://www.arjournals.org:80/index.php/ijdd/article/view/1031
 
11. Source Title; vol., no. (year) International Journal of Drug Delivery; Vol 5, No 2 (2013): International Journal of Drug Delivery
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c)