Colorimetric Method for the Estimation of Ethanol in Alcoholic-Drinks

Sandip V Sumbhate, Satish Nayak, Damodar Goupale, Ajay Tiwari, Rajesh S Jadon

Abstract


A method for estimating ethanol in alcoholic-drinks by direct reaction is presented. The method consist of color reaction of ethanol with sodium dichromate. The colorimetric quantification was based on the formation of green colored chromate ions resulting from treatment of ethanol and sodium dichromate as limiting reactant in presence of sulfuric acid and acetate buffer pH 4.3. The absorbance maxima for the ethanol was found to be 578 nm. The influence of acetate buffer pH, reaction time, and Beer’law on color development and sensitivity were investigated and optimal assay conditions established.

The limits of detection and quantification for ethanol were determined to be 0.6 mg/mL and 1.9 mg/mL.

References


Fleming M, Mithic JS, Harris AR. Ethanol, In Goodman & Gillman’s The Pharmacological Basis of Therapeutics,10th Ed.; .Limbard E. L., Hardman G. J. Eds.; McGraw-Hill, New Haven, CT, 2001, pp 429-430.

Johnson WA. Alcohols, Invitation to organic chemistry , Jones & Bartlett Publishers, subdury, MA, 1999, pp 138-139.

Stackler B. Christensen EN. Quantitative determination of ethanol in wine by Gas chromatography. Am J Enol Viticult. 1974;25:202-207.

Goel SC. Pamment NB. Direct injection technique for gas chromatographic determination of ethanol and other volatiles in concentrated cell suspensions. Biotec Let. 1984;6(4):177-182.

Lau O, Luk S. Spectrophotometric method for the determination of ethanol in beverages and beer samples using cerium(IV) as reagent. Int J Food Sci Technol. 1994;29(4):469-472

United States Pharmacopoeia and National Formulary (USP-30-NF-25) Vol 2. Rockville, M D: United States Pharmacopoeia Convention; 2007: 2525


Full Text: PDF
';

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

 

Advanced Research Journals

4/70-71. Black Well HB, AL 30100

Copyright@arjournals.org 2009-2011

 

Follow @arjournals on Twitter